• Login
    View Item 
    •   Maryland Shared Open Access Repository Home
    • ScholarWorks@UMBC
    • UMBC Interdepartmental Collections
    • UMBC Theses and Dissertations
    • View Item
    •   Maryland Shared Open Access Repository Home
    • ScholarWorks@UMBC
    • UMBC Interdepartmental Collections
    • UMBC Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Application of High Throughput Bioreactors for Subclone Selection

    Thumbnail
    Files
    302.pdf (918.8Kb)
    Permanent Link
    http://hdl.handle.net/11603/1057
    Collections
    • UMBC Chemical, Biochemical & Environmental Engineering Department
    • UMBC Graduate School
    • UMBC Student Collection
    • UMBC Theses and Dissertations
    Metadata
    Show full item record
    Author/Creator
    Drew, Jessica Lauren
    Date
    2008-10-22
    Type of Work
    application/pdf
    Text
    theses
    Department
    Chemical, Biochemical & Environmental Engineering
    Program
    Engineering
    Rights
    This item may be protected under Title 17 of the U.S. Copyright Law. It is made available by UMBC for non-commercial research and education. For permission to publish or reproduce, please see http://aok.lib.umbc.edu/specoll/repro.php or contact Special Collections at speccoll(at)umbc.edu.
    Access limited to the UMBC community. Item may possibly be obtained via Interlibrary Loan through a local library, pending author/copyright holder's permission.
    Subjects
    Engineering, Chemical(0542)
    subcloning
    mammalian cell culture
    improved protein productivity
    miniature bioreactors
    limiting dilution cloning
    Abstract
    The demand for monoclonal antibodies in pharmaceutical drug production requires the highest technology be invested in obtaining a stable, high producing cell line. Currently the most common method of selection is by limiting dilution cloning, done in well plates. The highest producing cell is chosen after samples from the stationary plates have been analyzed for antibody production. The selection is based on stationary culture, even though after scale-up cells will grow in a stirred environment. This research investigates a way to test multiple clones in a stirred environment by using high throughput bioreactors (HTBRs) in the early stages of clone selection. It has been found that simply selecting subclones based on results from stationary culture could result in the chance of missing even higher producing clones. Instead, choosing a clone after analyzing its performance in a stirred environment is an improved method to select a cell line for further scale-up.


    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3544


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.

     

     

    My Account

    LoginRegister

    Browse

    This CollectionBy Issue DateTitlesAuthorsSubjectsType

    Statistics

    View Usage Statistics


    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3544


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.