Mild Whole Body Hyperthermia Induced Interstitial Fluid Pressure (IFP) Reduction and Enhanced Nanoparticle Delivery to PC3 Tumors: In Vivo Studies and MicroCT Analyses
Loading...
Author/Creator ORCID
Date
2020-02-28
Type of Work
Department
Program
Citation of Original Publication
Gu, Qimei; Liu, Shuaishuai; Ray, Arunendra Saha; Florinas, Stelios; Christie, Ronald James; Daniel, Marie-Christine; Bieberich, Charles; Ma, Ronghui; Zhu, Liang; Mild Whole Body Hyperthermia Induced Interstitial Fluid Pressure (IFP) Reduction and Enhanced Nanoparticle Delivery to PC3 Tumors: In Vivo Studies and MicroCT Analyses; JOurnal of Thermal Science and Engineering Applications (1-23) (2020); https://asmedigitalcollection.asme.org/thermalscienceapplication/article-abstract/doi/10.1115/1.4046520/1074975/Mild-Whole-Body-Hyperthermia-Induced-Interstitial?redirectedFrom=fulltext
Rights
This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
Authors may reproduce and distribute the Paper for non-commercial purposes only. Non-commercial applies only to the sale of the paper per se. For all copies of the Paper made by Authors, Authors must acknowledge ASME as original publisher and include the names of all author(s), the publication title, and an appropriate copyright notice that identifies ASME as the copyright holder.
Access to this item will begin on 2021-02-28
Authors may reproduce and distribute the Paper for non-commercial purposes only. Non-commercial applies only to the sale of the paper per se. For all copies of the Paper made by Authors, Authors must acknowledge ASME as original publisher and include the names of all author(s), the publication title, and an appropriate copyright notice that identifies ASME as the copyright holder.
Access to this item will begin on 2021-02-28
Subjects
Abstract
In vivo experiments on mice were performed to evaluate whether whole body hyperthermia enhances nanoparticle delivery to PC3 tumors. The mice in the experimental group were subjected to whole body hyperthermia by maintaining their body temperatures at 40ºC for one hour. Interstitial fluid pressures (IFPs) in tumors were measured before heating, immediately after, and at 2 and 24 hours post-heating in both the experimental group and in a sham group (without heating). 0.2 cc of a newly developed nanofluid containing gold nanoparticles (AuNPs) was delivered via the tail vein in both groups. MicroCT scanned images of resected tumors were analyzed to visualize nanoparticle distribution in the tumors and to quantify the total amount of the nanoparticles delivered to the tumors. Statistically significant IFP reductions of 45% right after heating, 47% 2 hours post heating, and 52% 24 hours post heating were observed in the experimental group. Analyses of microCT scans of the resected tumors illustrated that nanoparticles were more concentrated near the tumor periphery rather than at the tumor center. The 1-hour whole body hyperthermia treatment resulted in more nanoparticles present in the tumor central region than that in the control group. The mass index calculated from the microCT scans suggested overall 42% more nanoparticle delivery in the experimental group than that in the control group. We conclude that 1-hour mild whole body hyperthermia leads to sustained reduction in tumoral IFPs and significantly increases the total amount of targeted gold nanoparticle deposition in PC3 tumors.