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dc.contributor.authorAlMatrouk, Abdullah
dc.contributor.authorLemons, Kayla
dc.contributor.authorOgura, Tatsuya
dc.contributor.authorLuo, Wangmei
dc.contributor.authorWilson, Chantel
dc.contributor.authorLin, Weihong
dc.date.accessioned2021-02-22T18:24:51Z
dc.date.available2021-02-22T18:24:51Z
dc.date.issued2018-09-27
dc.description.abstractFunctional maintenance of the mammalian main olfactory epithelium (MOE) is challenging because of its direct exposure to a wide spectrum of environmental chemicals. We previously reported that transient receptor potential channel M5-expressing microvillous cells (TRPM5-MCs) in the MOE play an important role in olfactory maintenance. To investigate the underpinning mechanisms, we exposed transcription factor Skn-1a knockout (Skn-1a−/−) mice lacking TRPM5-MCs, and TRPM5-GFP mice to either vehicle (water) or a mixture of odorous chemicals and chitin for two weeks and analyzed the expression of olfactory signaling proteins using immunolabeling and neurotrophin (NT) and NT receptor (NTR) gene transcripts using real-time quantitative PCR. The chemical exposure did not significantly attenuate the immunolabeling of olfactory signaling proteins. Vehicle-exposed Skn-1a−/− and TRPM5-GFP mice expressed similar levels of NT and NTR gene transcripts in the MOE and olfactory bulb. Chemical exposure significantly increased MOE expression of p75NTR in Skn-1a−/− mice, while p75NTR expression was reduced in TRPM5-GFP mice, as compared to vehicle-exposed mice. Additionally, our RNA in situ hybridization analysis and immunolabeling confirmed MOE expression of most NTs and NTRs. Together, these results indicate that TRPM5-MCs and chemical exposure influence expression of some NTs and NTRs in the MOE and olfactory bulb (OB).en_US
dc.description.sponsorshipWe thank Fenge Ni for technical assistance. We also thank Robert Margolskee and Ichiro Matsumoto for providing transgenic and knockout mice. This work was supported by a research grant (R01DC012831) to W. Lin from National Institute of Health/National Institute on Deafness and Other Communication Disorders in U.Sen_US
dc.description.urihttps://www.mdpi.com/1422-0067/19/10/2939en_US
dc.format.extent21 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m21750-uuyi
dc.identifier.citationAlMatrouk, Abdullah; Lemons, Kayla; Ogura, Tatsuya; Luo, Wangmei; Wilson, Chantel; Lin, Weihong. 2018. "Chemical Exposure-Induced Changes in the Expression of Neurotrophins and Their Receptors in the Main Olfactory System of Mice Lacking TRPM5-Expressing Microvillous Cells" Int. J. Mol. Sci. 19, no. 10: 2939. https://doi.org/10.3390/ijms19102939en_US
dc.identifier.urihttps://doi.org/10.3390/ijms19102939
dc.identifier.urihttp://hdl.handle.net/11603/21057
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleChemical Exposure-Induced Changes in the Expression of Neurotrophins and Their Receptors in the Main Olfactory System of Mice Lacking TRPM5-Expressing Microvillous Cellsen_US
dc.title.alternativeMice lacking transient receptor potential channel M5-expressing microvillous cells show different changes in the expression of neurotrophins and their receptors in the main olfactory system in response to chemical exposure
dc.typeTexten_US


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This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
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