Recent Submissions

  • Top-down Proteomic Characterization of Truncated Proteoforms 

    Chen, Dapeng; Geis-Asteggiante, Lucia; Gomes, Fabio; Ostrand-Rosenberg, Suzanne; Fenselau, Catherine (ACS Publications, 2019-09-10)
    A top-down proteomic strategy with semi-automated analysis of data sets has proven successful for global identification of truncated proteins without the use of chemical derivatization, enzymatic manipulation, immunoprecipitation ...
  • Effects of Lyse-It on endonuclease fragmentation, function and activity 

    Santaus, Tonya M.; Zhang, Fan; Li, Shan; Stine, O. Colin; Geddes, Chris D. (PLOS, 2019-09-30)
    Nucleases are enzymes that can degrade genomic DNA and RNA that decrease the accuracy of quantitative measures of those nucleic acids. Here, we study conventional heating, standard microwave irradiation, and Lyse-It, a ...
  • Assigning NMR spectra of RNA, peptides and small organic molecules using molecular network visualization software 

    Marchant, Jan; Summers, Michael F.; Johnson, Bruce A. (Springer Netherlands, 2019-07-19)
    NMR assignment typically involves analysis of peaks across multiple NMR spectra. Chemical shifts of peaks are measured before being assigned to atoms using a variety of methods. These approaches quickly become complicated ...
  • A comparison of Lyse-It to other cellular sample preparation, bacterial lysing, and DNA fragmentation technologies 

    Santaus, Tonya M.; Li, Shan; Saha, Lahari; Chen, Wilbur H.; Bhagat, Siya; Stine, O. Colin; Geddes, Chris D. (PLOS, 2019-07-23)
    The ability for safe and rapid pathogenic sample transportation and subsequent detection is an increasing challenge throughout the world. Herein, we describe and use bead-beating, vortex, sonication, 903 protein saver ...
  • Quantitative high-throughput screening assays for the discovery and development of SIRPα-CD47 interaction inhibitors 

    Miller, Thomas W.; Amason, Joshua D.; Garcin, Elsa D.; Lamy, Laurence; Dranchak, Patricia K.; Macarthur, Ryan; Braisted, John; Rubin, Jeffrey S.; Burgess, Teresa L.; Farrell, Catherine L.; Roberts, David D.; Inglese, James (PLOS, 2019-07-05)
    CD47 is an immune checkpoint molecule that downregulates key aspects of both the innate and adaptive anti-tumor immune response via its counter receptor SIRPα, and it is expressed at high levels in a wide variety of tumor ...
  • Escherichia Coli FeoC Binds a Redox-Active, Rapidly Oxygen-Sensitive [4Fe-4S] Cluster 

    Smith, Aaron T.; Linkous, Richard O.; Max, Nathan J.; Sestok, Alexandrea E.; Szalai, Veronika A.; Chacón, Kelly N. (2019-02-07)
    The acquisition of iron is essential to establishing virulence among most pathogens. Under acidic and/or anaerobic conditions, most bacteria utilize the widely-distributed ferrous iron (Fe²⁺) uptake (Feo) system to import ...
  • Distorted perovskites for high voltage dielectric capacitors 

    Gill, Puneet; Bagherzadeh, Hedyeh; Choa, Fow-Sen; Arnold, Bradley; Smith, Paul; Su, Ching Hua; Verma, Manish; Singh, Laxman; Mandal, Kamdeo; Singh, N. B. (SPIE, 2019-05-13)
    There are several mechanisms which have been proposed for the existence of colossal dielectric constant in the class of perovskite calcium copper titanate (CaCu₃Ti₄O₁₂ or CCTO) materials. Researches indicate that existence ...
  • Design of materials for IR detectors using high Z elements for high energy radiation environment 

    Saraf, Sonali; Cooper, Christopher; Su, Ching Hua; Choa, Fow-Sen; Arnold, Bradley; Cullum, Brian; Sachs, David; DiPaula, Philip; Singh, N. B. (SPIE, 2019-05-13)
    There is a strong need for rad hard and high operating temperature IR detectors for space environment. Heavy metal Selenides (high Z and large density) have been investigated for more than half century for high operating ...
  • The Crystal Structure of Klebsiella pneumoniae FeoA Reveals a Site For Protein-Protein Interactions 

    Linkous, Richard O.; Sestok, Alexandrea E.; Smith, Aaron T. (2019-01-08)
    In order to establish infection, pathogenic bacteria must obtain essential nutrients such as iron. Under acidic and/or anaerobic conditions, most bacteria utilize the Feo system in order to acquire ferrous iron (Fe²⁺) from ...
  • Replicating Meyerhoff for inclusive excellence in STEM 

    Sto. Domingo, Mariano R.; Sharp, Starlette; Freeman, Amy; Freeman Jr., Thomas; Freeman, Thomas, Jr.; Harmon, Keith; Wiggs, Mitsue; Sathy, Viji; Panter, Abigail T.; Oseguera, Leticia; Sun, Shuyan; Williams, Mary Elizabeth; Templeton, Joseph; Folt, Carol L.; Barron, Eric J.; Hrabowski III, Freeman A.; Maton, Kenneth I.; Crimmins, Michael; Fisher, Charles R.; Summers, Michael F. (American Association for the Advancement of Science, 2019-04-26)
  • Importance of lotus effect on surface sensing 

    Sova, Stacey; Prasad, Narasimha; Cooper, Christopher; Kelly, Lisa; Arnold, Bradley; Cullum, Brian; Choa, Fow-Sen; Singh, N. B. (SPIE, 2019-05-02)
    Superhydrophobic polymer films are a material of interest for aircraft deicing fluids to achieve the selfcleaning lotus effect. Hydrophobic polymer films were obtained by a solvent selective method composed of hydrophilic ...
  • Thermally-induced optical reflection of sound (THORS) for photoacoustic sensing 

    Kazal, Daniel S.; Holthoff, Ellen L.; Cullum, Brian M. (SPIE, 2019-05-02)
    The ability to precisely control and manipulate acoustic waves can be highly limiting in applications and environments where placement of physical barriers for acoustic steering cannot be employed (e.g. tissues, air, etc.) ...
  • Dielectric properties of CaCu₃₋ₓMgₓTi₄O₁₂ (x=0.20 and 0.50) material synthesized by the semi-wet route for energy storage capacitor 

    Singh, Laxman; Rai, U. S.; Singh, N. B.; Lee, Y.; Mahato, D. K. (SPIE, 2019-05-02)
    Dielectric properties of high percentage Mg doped CaCu₃Ti₄O₁₂, CaCu₃-ₓMgₓTi₄O₁₂ (x=0.20 and 0.50) ceramics carried out. They were synthesized by the semi-wet route using a solution based citrate–nitrate method along with ...
  • Effect of processing on morphology of hydroxyapatites: bioactive glasses and crystalline composites 

    McAdams, Joel; Bowman, Eric; Cullum, Brian; Arnold, Bradley; Kelly, Lisa; Choa, Fow Sen; Singh, N. B.; Su, Ching Hua; Mandal, K. D.; Singh, Shruti (SPIE, 2019-05-02)
    Recent studies on multinary oxides for applications as laser hosts and high dielectric capacitors have shown that processing at high temperature provides glassy or crystalline materials based on thermal treatments and ...
  • Microchip-based 3D-Cell Culture Using Polymer Nanofibers Generated by Solution Blow Spinning 

    Chen, Chengpeng; Townsend, Alexandra D.; Sell, Scott A.; Martin, R. Scott (Royal Society of Chemistry, 2017-04-21)
    Polymer nano/micro fibers have found many applications including 3D cell culture and the creation of wound dressings. The fibers can be produced by a variety of techniques that include electrospinning, the primary disadvantage ...
  • Insert-based Microfluidics for 3D Cell Culture with Analysis 

    Chen, Chengpeng; Townsend, Alexandra D.; Hayter, Elizabeth A.; Birk, Hannah M.; Sell, Scott A.; Martin, R. Scott (Springer Berlin Heidelberg, 2018-03-14)
    We present an insert-based approach to fabricate scalable and multiplexable microfluidic devices for 3D cell culture and integration with downstream detection modules. Laser-cut inserts with a layer of electrospun fibers ...
  • A review of electrospinning manipulation techniques to direct fiber deposition and maximize pore size 

    Feltz, Kevin P.; Growney Kalaf, Emily A.; Chen, Chengpeng; Martin, R. Scott; Sell, Scott A. (Walter de Gruyter GmbH, 2017-06-18)
    Electrospinning has been widely accepted for several decades by the tissue engineering and regenerative medicine community as a technique for nanofiber production. Owing to the inherent flexibility of the electrospinning ...
  • Use of Electrospinning and Dynamic Air Focusing to Create Three-Dimensional Cell Culture Scaffolds in Microfluidic Devices 

    Chen, Chengpeng; Mehl, Benjamin T.; Sell, Scott A.; Martin, R. Scott (Royal Society of Chemistry, 2016-07-04)
    Organs-on-a-chip has emerged as a powerful tool for pharmacological and physiological studies. A key part in the construction of such a model is the ability to pattern or culture cells in a biomimetic fashion. Most of the ...
  • Enzyme Modification and Oxidative Crosslinking using Carboxylate-, Phenol-and Catechol-Conjugated 1,8-Naphthalimides 

    Sova, Stacey; Kelly, Lisa A. (John Wiley & Sons, 2019-04-16)
    The ground‐ and excited‐state interactions of β‐alanine, tyrosine and L‐dopa substituted 1,8 naphthalimides (NI‐Ala, NI‐Tyr and NI‐Dopa) with lysozyme and mushroom tyrosinase were evaluated to understand the mechanism of ...
  • Using Clickers for Deliberate Practice in Five Large Science Courses 

    Hodges, Linda C.; Anderson, Eric C.; Carpenter, Tara S.; Cui, Lili; Feeser, Elizabeth A.; Gierasch, Tiffany Malinky (NSTA, 2017-11-01)
    Clickers are often used as an active learning tool in face-to-face classes to enhance student engagement and assess student learning. In this article we share the variety of ways that we use clicker questions ...

View more