Nonspecific Deposition Of IL-8 By Human Neutrophils During Chemotaxis In Vitro
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Date
2005-11
Department
Hood College Biology
Program
Biomedical and Environmental Science
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Abstract
Polymorphonuclear neutrophils (PMNs) of the innate immune system are
responsible for ingestion and killing of invading microorganisms. However, microbial
clearance by PMNs requires chemotaxis along a chemoattractant gradient from the
vasculature to an infectious focus. Shown here, treatment of PMNs with fibrinogen
enhances migration to the chemoattractant, formyl-methionyl-leucyl-phenylalanine
(fMLF), increasing the number of migrating PMNs and migratory distance. Previous
studies demonstrated that fibrinogen enhanced MU-stimulated production of another
chemoattractant, interleukin-8 (IL-8). IL-8, released by migrating PMNs, could provide
an additional chemoattractant for the further migration of additional PMNs, amplifying
the inflammatory response. The cationic nature of IL-8 could facilitate its
immobilization to the extracellular matrix into chemoattractant "trails." Using "underagarose"
chemotaxis and amplified immunofluorescence staining, areas of extracellular
IL-8 staining were identified; however, instead of specific deposition of IL-8, these areas
appeared to result from nonspecific deposition of cellular debris since they contained
other cellular components such as B-actin.