Nonspecific Deposition Of IL-8 By Human Neutrophils During Chemotaxis In Vitro

dc.contributor.authorFink, Danielle
dc.contributor.departmentHood College Biology
dc.contributor.programBiomedical and Environmental Science
dc.date.accessioned2023-12-01T13:26:49Z
dc.date.available2023-12-01T13:26:49Z
dc.date.issued2005-11
dc.description.abstractPolymorphonuclear neutrophils (PMNs) of the innate immune system are responsible for ingestion and killing of invading microorganisms. However, microbial clearance by PMNs requires chemotaxis along a chemoattractant gradient from the vasculature to an infectious focus. Shown here, treatment of PMNs with fibrinogen enhances migration to the chemoattractant, formyl-methionyl-leucyl-phenylalanine (fMLF), increasing the number of migrating PMNs and migratory distance. Previous studies demonstrated that fibrinogen enhanced MU-stimulated production of another chemoattractant, interleukin-8 (IL-8). IL-8, released by migrating PMNs, could provide an additional chemoattractant for the further migration of additional PMNs, amplifying the inflammatory response. The cationic nature of IL-8 could facilitate its immobilization to the extracellular matrix into chemoattractant "trails." Using "underagarose" chemotaxis and amplified immunofluorescence staining, areas of extracellular IL-8 staining were identified; however, instead of specific deposition of IL-8, these areas appeared to result from nonspecific deposition of cellular debris since they contained other cellular components such as B-actin.
dc.format.extent54 pages
dc.genreThesis
dc.identifier.urihttp://hdl.handle.net/11603/30989
dc.language.isoen_US
dc.titleNonspecific Deposition Of IL-8 By Human Neutrophils During Chemotaxis In Vitro
dc.typeText

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