Nonspecific Deposition Of IL-8 By Human Neutrophils During Chemotaxis In Vitro
dc.contributor.author | Fink, Danielle | |
dc.contributor.department | Hood College Biology | |
dc.contributor.program | Biomedical and Environmental Science | |
dc.date.accessioned | 2023-12-01T13:26:49Z | |
dc.date.available | 2023-12-01T13:26:49Z | |
dc.date.issued | 2005-11 | |
dc.description.abstract | Polymorphonuclear neutrophils (PMNs) of the innate immune system are responsible for ingestion and killing of invading microorganisms. However, microbial clearance by PMNs requires chemotaxis along a chemoattractant gradient from the vasculature to an infectious focus. Shown here, treatment of PMNs with fibrinogen enhances migration to the chemoattractant, formyl-methionyl-leucyl-phenylalanine (fMLF), increasing the number of migrating PMNs and migratory distance. Previous studies demonstrated that fibrinogen enhanced MU-stimulated production of another chemoattractant, interleukin-8 (IL-8). IL-8, released by migrating PMNs, could provide an additional chemoattractant for the further migration of additional PMNs, amplifying the inflammatory response. The cationic nature of IL-8 could facilitate its immobilization to the extracellular matrix into chemoattractant "trails." Using "underagarose" chemotaxis and amplified immunofluorescence staining, areas of extracellular IL-8 staining were identified; however, instead of specific deposition of IL-8, these areas appeared to result from nonspecific deposition of cellular debris since they contained other cellular components such as B-actin. | |
dc.format.extent | 54 pages | |
dc.genre | Thesis | |
dc.identifier.uri | http://hdl.handle.net/11603/30989 | |
dc.language.iso | en_US | |
dc.title | Nonspecific Deposition Of IL-8 By Human Neutrophils During Chemotaxis In Vitro | |
dc.type | Text |