Bioburden detection on surface and water samples in a rapid, ultra-sensitive and high-throughput manner

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Bioburden_detection_on_surface_and_water_samples.pdf (735.22 KB)

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https://onlinelibrary.wiley.com/doi/abs/10.1002/btpr.3457

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https://doi.org/10.1002/btpr.3457
 http://hdl.handle.net/11603/33012

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UMBC Faculty Collection
UMBC Center for Advanced Sensor Technology (CAST)
UMBC Chemical, Biochemical & Environmental Engineering Department
UMBC Computer Science and Electrical Engineering Department
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Author/Creator ORCID

https://orcid.org/0000-0002-4344-6147
 https://orcid.org/0000-0003-1733-398X

Date

2024-03-17

Type of Work

journal articles
postprints
Text

Department

Program

Citation of Original Publication

Hasan, Md Sadique, Chad Sundberg, Elias Gilotte, Xudong Ge, Yordan Kostov, and Govind Rao. “Bioburden Detection on Surface and Water Samples in a Rapid, Ultra-Sensitive and High-Throughput Manner.” Biotechnology Progress (17 March 2024): e3457. https://doi.org/10.1002/btpr.3457.

Rights

This is the peer reviewed version of the following article: Hasan, Md Sadique, Chad Sundberg, Elias Gilotte, Xudong Ge, Yordan Kostov, and Govind Rao. “Bioburden Detection on Surface and Water Samples in a Rapid, Ultra-Sensitive and High-Throughput Manner.” Biotechnology Progress (17 March 2024): e3457. https://doi.org/10.1002/btpr.3457., which has been published in final form at https://doi.org/10.1002/btpr.3457. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Access to this item will begin on 03-17-2025.

Subjects

assay
bioburden
contamination detection
multichannel fluorometer
plate count
resazurin
TSB

Abstract

Bioburden detection is crucial for food, water, and biopharmaceutical applications as it can directly impact public health. The objective of this study is to develop and validate an assay and protocol for detecting bioburden on solid surfaces, as well as in water, with high sensitivity and accuracy in a rapid manner. Henceforth, a resazurin-based assay optimized for detecting bioburden has been integrated with a previously developed portable multichannel fluorometer. The microbes were isolated from solid surfaces in different laboratory settings by swabbing technique, and stream water was collected for contamination analysis. Based on the results, the assay and protocol can successfully detect bioburden as low as 20 CFU/cm² and 10 CFU/mL present in both surface and water samples, respectively.