Bioburden detection on surface and water samples in a rapid, ultra-sensitive and high-throughput manner

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dc.contributor.authorHasan, Md Sadique
dc.contributor.authorSundberg, Chad Alan
dc.contributor.authorGilotte, Elias
dc.contributor.authorGe, Xudong
dc.contributor.authorKostov, Yordan
dc.contributor.authorRao, Govind
dc.date.accessioned2024-04-10T19:05:54Z
dc.date.available2024-04-10T19:05:54Z
dc.date.issued2024-03-17
dc.description.abstractBioburden detection is crucial for food, water, and biopharmaceutical applications as it can directly impact public health. The objective of this study is to develop and validate an assay and protocol for detecting bioburden on solid surfaces, as well as in water, with high sensitivity and accuracy in a rapid manner. Henceforth, a resazurin-based assay optimized for detecting bioburden has been integrated with a previously developed portable multichannel fluorometer. The microbes were isolated from solid surfaces in different laboratory settings by swabbing technique, and stream water was collected for contamination analysis. Based on the results, the assay and protocol can successfully detect bioburden as low as 20 CFU/cm² and 10 CFU/mL present in both surface and water samples, respectively.
dc.description.sponsorshipThis work was supported by the U.S. Food and Drug Administration under contract no. BAA 75F40119C10132
dc.description.urihttps://onlinelibrary.wiley.com/doi/abs/10.1002/btpr.3457
dc.format.extent15 pages
dc.genrejournal articles
dc.genrepostprints
dc.identifierdoi:10.13016/m2bmzd-k8xi
dc.identifier.citationHasan, Md Sadique, Chad Sundberg, Elias Gilotte, Xudong Ge, Yordan Kostov, and Govind Rao. “Bioburden Detection on Surface and Water Samples in a Rapid, Ultra-Sensitive and High-Throughput Manner.” Biotechnology Progress (17 March 2024): e3457. https://doi.org/10.1002/btpr.3457.
dc.identifier.urihttps://doi.org/10.1002/btpr.3457
dc.identifier.urihttp://hdl.handle.net/11603/33012
dc.language.isoen_US
dc.publisherWiley
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Center for Advanced Sensor Technology (CAST)
dc.relation.ispartofUMBC Computer Science and Electrical Engineering Department
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department
dc.relation.ispartofUMBC Student Collection
dc.rightsThis is the peer reviewed version of the following article: Hasan, Md Sadique, Chad Sundberg, Elias Gilotte, Xudong Ge, Yordan Kostov, and Govind Rao. “Bioburden Detection on Surface and Water Samples in a Rapid, Ultra-Sensitive and High-Throughput Manner.” Biotechnology Progress (17 March 2024): e3457. https://doi.org/10.1002/btpr.3457., which has been published in final form at https://doi.org/10.1002/btpr.3457. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
dc.rightsAccess to this item will begin on 03-17-2025.
dc.subjectassay
dc.subjectbioburden
dc.subjectcontamination detection
dc.subjectmultichannel fluorometer
dc.subjectplate count
dc.subjectresazurin
dc.subjectTSB
dc.titleBioburden detection on surface and water samples in a rapid, ultra-sensitive and high-throughput manner
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0002-4344-6147
dcterms.creatorhttps://orcid.org/0000-0003-1733-398X

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