Application of High Throughput Bioreactors for Subclone Selection

Author/Creator ORCID




Chemical, Biochemical & Environmental Engineering



Citation of Original Publication


This item may be protected under Title 17 of the U.S. Copyright Law. It is made available by UMBC for non-commercial research and education. For permission to publish or reproduce, please see or contact Special Collections at speccoll(at)
Access limited to the UMBC community. Item may possibly be obtained via Interlibrary Loan through a local library, pending author/copyright holder's permission.


The demand for monoclonal antibodies in pharmaceutical drug production requires the highest technology be invested in obtaining a stable, high producing cell line. Currently the most common method of selection is by limiting dilution cloning, done in well plates. The highest producing cell is chosen after samples from the stationary plates have been analyzed for antibody production. The selection is based on stationary culture, even though after scale-up cells will grow in a stirred environment. This research investigates a way to test multiple clones in a stirred environment by using high throughput bioreactors (HTBRs) in the early stages of clone selection. It has been found that simply selecting subclones based on results from stationary culture could result in the chance of missing even higher producing clones. Instead, choosing a clone after analyzing its performance in a stirred environment is an improved method to select a cell line for further scale-up.