Hydroporphyrin-Doped Near-Infrared-Emitting Polymer Dots for Cellular Fluorescence Imaging

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dc.contributor.authorRiahin, Connor
dc.contributor.authorMeares, Adam
dc.contributor.authorEsemoto, Nopondo Ndoh
dc.contributor.authorPtaszek, Marcin
dc.contributor.authorLaScola, Michael
dc.contributor.authorPandala, Narendra
dc.contributor.authorLavik, Erin
dc.contributor.authorYang, Mengran
dc.contributor.authorStacey, Gary
dc.contributor.authorHu, Dehong
dc.contributor.authorTraeger, Jeremiah C.
dc.contributor.authorOrr, Galya
dc.contributor.authorRosenzweig, Zeev
dc.date.accessioned2022-05-27T20:36:26Z
dc.date.available2022-05-27T20:36:26Z
dc.date.issued2022-04-22
dc.description.abstractNear-infrared (NIR) fluorescent semiconductor polymer dots (Pdots) have shown great potential for fluorescence imaging due to their exceptional chemical and photophysical properties. This paper describes the synthesis of NIR-emitting Pdots with great control and tunability of emission peak wavelength. The Pdots were prepared by doping poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo-(2,1′,3)-thiadiazole)] (PFBT), a semiconducting polymer commonly used as a host polymer in luminescent Pdots, with a series of chlorins and bacteriochlorins with varying functional groups. Chlorins and bacteriochlorins are ideal dopants due to their high hydrophobicity, which precludes their use as molecular probes in aqueous biological media but on the other hand prevents their leakage when doped into Pdots. Additionally, chlorins and bacteriochlorins have narrow deep red to NIR-emission bands and the wide array of synthetic modifications available for modifying their molecular structure enables tuning their emission predictably and systematically. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) measurements show the chlorin- and bacteriochlorin-doped Pdots to be nearly spherical with an average diameter of 46 ± 12 nm. Efficient energy transfer between PFBT and the doped chlorins or bacteriochlorins decreases the PFBT donor emission to near baseline level and increases the emission of the doped dyes that serve as acceptors. The chlorin- and bacteriochlorin-doped Pdots show narrow emission bands ranging from 640 to 820 nm depending on the doped dye. The paper demonstrates the utility of the systematic chlorin and bacteriochlorin synthesis approach by preparing Pdots of varying emission peak wavelength, utilizing them to visualize multiple targets using wide-field fluorescence microscopy, binding them to secondary antibodies, and determining the binding of secondary antibody-conjugated Pdots to primary antibody-labeled receptors in plant cells. Additionally, the chlorin- and bacteriochlorin-doped Pdots show a blinking behavior that could enable their use in super-resolution imaging methods like STORM.en_US
dc.description.sponsorshipThe study is primarily supported by the US Department of Energy Basic Energy Research (DOE-BER) grant DE-SC0020346. C.R.’s and N.N.E’s doctoral studies are partially supported by NIH Chemistry–Biology Interface (CBI) training grant NIH/NIGMS T32 GM066706. N.N.E is also partially supported by the Meyerhoff Graduate Program at UMBC, which is supported by NIH-NIGMS iniative for maximizing student development (Grant No. 2 R25-GM55036). Postdoctoral support for M.Y. was provided by a grant from the National Science Foundation Plant Genome Program (Grant No. IOS-2048410). A portion of this research was performed at the Environmental Molecular Sciences Laboratory, a DOE Office of Science User Facility sponsored by the Biological and Environmental Research program under Contract No. DE-AC05-76RL01830.en_US
dc.description.urihttps://pubs.acs.org/doi/10.1021/acsami.2c02551en_US
dc.format.extent12 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2ygem-fj9p
dc.identifier.citationRiahin, Connor. Hydroporphyrin-Doped Near-Infrared-Emitting Polymer Dots for Cellular Fluorescence Imaging. ACS Applied Materials & Interfaces. Apr. 8 2022. DOI: 10.1021/acsami.2c02551en_US
dc.identifier.urihttps://doi.org/10.1021/acsami.2c02551
dc.identifier.urihttp://hdl.handle.net/11603/24755
dc.language.isoen_USen_US
dc.publisherACSen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemistry & Biochemistry Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department
dc.relation.ispartofUMBC College of Engineering and Information Technology Dean's Office
dc.rightsThis work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.en_US
dc.rightsPublic Domain Mark 1.0*
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/*
dc.titleHydroporphyrin-Doped Near-Infrared-Emitting Polymer Dots for Cellular Fluorescence Imagingen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0001-5212-4484en_US
dcterms.creatorhttps://orcid.org/0000-0001-6468-6900en_US
dcterms.creatorhttps://orcid.org/0000-0002-3057-8327en_US
dcterms.creatorhttps://orcid.org/0000-0002-0644-744Xen_US
dcterms.creatorhttps://orcid.org/0000-0001-6098-3932en_US

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