Targeted K-Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CT
dc.contributor.author | Gunaseelan, Nivetha | |
dc.contributor.author | Moitra, Parikshit | |
dc.contributor.author | Saha, Pranay | |
dc.contributor.author | Aditya, Teresa | |
dc.contributor.author | Moghiseh, Mahdieh | |
dc.contributor.author | Jonker, Kevin | |
dc.contributor.author | Gieseg, Steven | |
dc.contributor.author | Butler, Anthony | |
dc.contributor.author | Kamal, Fadia | |
dc.contributor.author | Pan, Prof Dipanjan | |
dc.date.accessioned | 2024-11-14T15:18:40Z | |
dc.date.available | 2024-11-14T15:18:40Z | |
dc.date.issued | 2024-10-07 | |
dc.description.abstract | Utilizing metal nanoprobes with unique K-edge identities to visualize complementary biological activities simultaneously can provide valuable information about complex biological processes. This study describes the design and preparation of an innovative pair of K-edge metal nanoprobes and demonstrates the feasibility of their simultaneous quantitative detection using spectral photon-counting computed tomography (SPCCT). Glycosaminoglycan (GAG) capped nanoparticles (ca. 15–20 nm) targeting two distinct components of the cartilage tissue, namely, aggrecan (acan) and aggrecanase (acanase) are designed and synthesized. These targeted nanoparticles comprised of praseodymium (Pr) and hafnium (Hf), with well-separated K-edge energies, enable simultaneous and ratiometric imaging of dual biomarkers in cartilage tissue. Following extensive physico-chemical characterization of the ligand-targeted particles, the feasibility of homing dual biomarkers in vitro is demonstrated. The material discrimination and simultaneous quantification of these targeted particles are also achieved and corroborated with inductively coupled plasmon spectroscopy. For the first time, the use of praseodymium is reported as a contrast agent for SPCCT imaging and demonstrates the ability to pair it with hafnium nanoprobes for multicontrast imaging of diseases. Importantly, the potential for ratiometric molecular imaging and tracking of osteoarthritis (OA) progression is shown with SPCCT K-edge based imaging approach. | |
dc.description.sponsorship | N.G. and P.M. contributed equally to this work as first authors. P.S. and T.A. contributed equally as second authors. The authors gratefully acknowledge the support of the Pennsylvania State University and Huck Institute of Life Sciences at the Pennsylvania State University in completing this project. The authors also gratefully acknowledge the support from the Center for Infectious Disease Dynamics at the Pennsylvania State University. D.P. acknowledges funding from the Centers for Disease Control and Prevention (Award No. 75D30122C15492), the National Science Foundation (Award No. CBET 2153091/2229986), and the Department of Defense (DoD) Congressionally Directed Medical Research Programs (CDMRP) (Award No. TP210376). The cartoons in the figures were created with BioRender.com. | |
dc.description.uri | https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202408408 | |
dc.format.extent | 16 pages | |
dc.genre | journal articles | |
dc.identifier | doi:10.13016/m2t9jr-xkmm | |
dc.identifier.citation | Gunaseelan, Nivetha, Parikshit Moitra, Pranay Saha, Teresa Aditya, Mahdieh Moghiseh, Kevin Jonker, Steven Gieseg, Anthony Butler, Fadia Kamal, and Dipanjan Pan. “Targeted K-Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers Using Spectral Photon Counting CT.” Advanced Science., 2408408. https://doi.org/10.1002/advs.202408408. | |
dc.identifier.uri | https://doi.org/10.1002/advs.202408408 | |
dc.identifier.uri | http://hdl.handle.net/11603/36949 | |
dc.language.iso | en_US | |
dc.publisher | Wiley | |
dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
dc.relation.ispartof | UMBC Student Collection | |
dc.relation.ispartof | UMBC Chemical, Biochemical & Environmental Engineering Department | |
dc.relation.ispartof | UMBC Faculty Collection | |
dc.rights | Attribution 4.0 International CC BY 4.0 Deed | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | spectral photon counting computed tomography | |
dc.subject | praseodymium nanoparticles | |
dc.subject | K-edge imaging | |
dc.subject | osteoarthritis tracking | |
dc.subject | quantitative imaging | |
dc.subject | hafnium nanoparticles | |
dc.title | Targeted K-Edge Nanoprobes From Praseodymium and Hafnium for Ratiometric Tracking of Dual Biomarkers using Spectral Photon Counting CT | |
dc.type | Text | |
dcterms.creator | https://orcid.org/0000-0003-4939-1658 | |
dcterms.creator | https://orcid.org/0000-0003-0175-4704 |