Reconstitution of the Arginyltransferase (ATE₁) Iron-Sulfur Cluster

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dc.contributor.authorVan, Verna
dc.contributor.authorSmith, Aaron T.
dc.date.accessioned2023-05-18T17:19:10Z
dc.date.available2023-05-18T17:19:10Z
dc.date.issued2023-04-04
dc.description.abstractAs global regulators of eukaryotic homeostasis, arginyltransferases (ATE1s) have essential functions within the cell. Thus, the regulation of ATE1 is paramount. It was previously postulated that ATE1 was a hemoprotein and that heme was an operative cofactor responsible for enzymatic regulation and inactivation. However, we have recently shown that ATE1 instead binds an iron-sulfur ([Fe-S]) cluster that appears to function as an oxygen sensor to regulate ATE1 activity. As this cofactor is oxygen-sensitive, purification of ATE1 in the presence of O₂ results in cluster decomposition and loss. Here we describe an anoxic chemical reconstitution protocol to assemble the [Fe-S] cluster cofactor in Saccharomyces cerevisiae ATE1 (ScATE1) and Mus musculus ATE1 isoform 1 (MmATE1-1).en_US
dc.description.sponsorshipThis work was supported by NIH-NIGMS grant R35 GM133497 (A.T.S).en_US
dc.description.urihttps://link.springer.com/protocol/10.1007/978-1-0716-2942-0_23en_US
dc.format.extent20 pagesen_US
dc.genrebook chaptersen_US
dc.genrepostprintsen_US
dc.identifierdoi:10.13016/m2osph-vmkk
dc.identifier.citationVan, V., Smith, A.T. (2023). Reconstitution of the Arginyltransferase (ATE₁) Iron-Sulfur Cluster. In: Kashina, A.S. (eds) Protein Arginylation. Methods in Molecular Biology, vol 2620. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2942-0_23.en_US
dc.identifier.urihttps://doi.org/10.1007/978-1-0716-2942-0_23
dc.identifier.urihttp://hdl.handle.net/11603/28018
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemistry & Biochemistry Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.rightsAccess to this item will begin on 04/04/2025
dc.titleReconstitution of the Arginyltransferase (ATE₁) Iron-Sulfur Clusteren_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0002-6605-9764en_US
dcterms.creatorhttps://orcid.org/0000-0002-9332-8683en_US

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