17β-Estradiol (E2) may be involved in the mode of crustacean female sex hormone (CFSH) action in the blue crab, Callinectes sapidus
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2022-07-25
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Wang T, He K, Blaney L and Chung JS (2022) 17β-Estradiol (E2) may be involved in the mode of crustacean female sex hormone (CFSH) action in the blue crab, Callinectes sapidus. Front. Endocrinol. 13:962576. doi: 10.3389/fendo.2022.962576
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Attribution 4.0 International (CC BY 4.0)
Attribution 4.0 International (CC BY 4.0)
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Abstract
17β-estradiol (E2) has been proved to control reproduction, sexual
differentiation, and the development of the secondary sexual characteristics
of vertebrate females. In decapod crustacean species, crustacean female sex
hormone (CFSH), a protein hormone, is required for developing adult-specific
ovigerous setae for embryo brooding and gonophores for mating at the blue
crab Callinectes sapidus puberty molting. However, it is unclear that whether
the mode of CFSH action involves a vertebrate-type sex steroid hormone in
crustaceans. To this end, E2 levels were first measured using a competitive
ELISA in the hemolymph and the potential CFSH target tissues from both
prepuberty and adult females; the presence of E2 was further confirmed with a
liquid chromatography tandem mass spectrometry method. Then, the cDNAs
of the following genes known to be associated with vertebrate steroidogenic
pathways were isolated: StAR-related lipid transfer protein 3 (StAR3); 3β-hydroxysteroid dehydrogenase (3βHSD); two isoforms of 17β-hydroxysteroid
dehydrogenase 8 (17βHSD8); and, estradiol-related receptor (ERR). RT-PCR
analysis revealed that these genes were widely distributed in the eyestalk
ganglia, hepatopancreas, brain, ovary, spermathecae, ovigerous and plumose
setae tissues of adult females. The 17βHSD8 transcripts were localized in the
follicle cells, the periphery of the nuclear membrane of primary oocytes, and
yolk granules of the vitellogenic oocytes using in situ hybridization, and the
corresponding protein was detected in the follicle cells and ooplasm of primary
oocytes using immunohistochemistry. Furthermore, the adult females injected
with CFSH-dsRNA (n = 30 times) had E2 and StAR3 transcripts levels lower in
the ovigerous and plumose setae, spermathecae than controls. These results
suggested that the mode of CFSH action in C. sapidus might involve E2 in these
adult-female-specific tissues.