Aspergillus nidulans Septa Are Indispensable for Surviving Cell Wall Stress

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dc.contributor.authorSpence, Ryland N
dc.contributor.authorHuso, Walker
dc.contributor.authorEdwards, Harley
dc.contributor.authorDoan, Alexander
dc.contributor.authorReese, Samantha
dc.contributor.authorHarris, Steven D.
dc.contributor.authorSrivastava, Ranjan
dc.contributor.authorMarten, Mark
dc.date.accessioned2022-03-07T15:47:45Z
dc.date.available2022-03-07T15:47:45Z
dc.date.issued2022-02-02
dc.description.abstractSeptation in filamentous fungi is a normal part of development, which involves the formation of cross-hyphal bulkheads, typically containing pores, allowing cytoplasmic streaming between compartments. Based on previous findings regarding septa and cell wall stress, we hypothesized that septa are critical for survival during cell wall stress. To test this hypothesis, we used known Aspergillus nidulans septation-deficient mutants (ΔsepH, Δbud3, Δbud4, and Δrho4) and six antifungal compounds. Three of these compounds (micafungin, Congo red, and calcofluor white) are known cell wall stressors which activate the cell wall integrity signaling pathway (CWIS), while the three others (cycloheximide, miconazole, and 2,3-butanedione monoxime) perturb specific cellular processes not explicitly related to the cell wall. Our results show that deficiencies in septation lead to fungi which are more susceptible to cell wall-perturbing compounds but are no more susceptible to other antifungal compounds than a control. This implies that septa play a critical role in surviving cell wall stress.en_US
dc.description.sponsorshipThis material is based upon work supported by the National Science Foundation under grant no. 2006189 (UMBC) and 2006190 (U. Conn). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.en_US
dc.description.urihttps://journals.asm.org/doi/10.1128/spectrum.02063-21en_US
dc.format.extent5 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2gdud-vqp9
dc.identifier.citationSpence, Ryland N. et al. Aspergillus nidulans Septa Are Indispensable for Surviving Cell Wall Stress. Microbiology Spectrum (Feb. 2, 2022). https://doi.org/10.1128/spectrum.02063-21.en_US
dc.identifier.urihttps://doi.org/10.1128/spectrum.02063-21
dc.identifier.urihttp://hdl.handle.net/11603/24351
dc.language.isoen_USen_US
dc.publisherASMen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleAspergillus nidulans Septa Are Indispensable for Surviving Cell Wall Stressen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0002-1863-8956en_US

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