Mind bomb 2 promotes cell migration and epithelial structure by regulating adhesion complexes and the actin cytoskeleton

Thumbnail Image

Files

Trivedi-accepted-2022.pdf (8.38 MB)
1-s2.0-S0012160622001610-mmc1.docx (4.95 MB)
1-s2.0-S0012160622001610-mmc2.xlsx (17.69 KB)

Links to Files

https://www.sciencedirect.com/science/article/pii/S0012160622001610?via%3Dihub

Permanent Link

https://doi.org/10.1016/j.ydbio.2022.08.009
 http://hdl.handle.net/11603/26191

Collections

UMBC Biological Sciences Department
UMBC Faculty Collection
UMBC Student Collection

Author/Creator

Author/Creator ORCID

https://orcid.org/0000-0002-9840-9028
 https://orcid.org/0000-0002-0855-0715

Date

2022-09-17

Type of Work

journal articles
postprints
Text

Department

Program

Citation of Original Publication

Trivedi, Sunny; Mallika Bhattacharya and Michelle Starz-Gaiano." Mind bomb 2 promotes cell migration and epithelial structure by regulating adhesion complexes and the actin cytoskeleton." Developmental Biology 491 (17 September 2022): 94-104. https://doi.org/10.1016/j.ydbio.2022.08.009.

Rights

This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access to this item will begin on 09-17-2023

Subjects

Abstract

Cell migration is essential in animal development and co-opted during metastasis and inflammatory diseases. Some cells migrate collectively, which requires them to balance epithelial characteristics such as stable cell-cell adhesions with features of motility like rapid turnover of adhesions and dynamic cytoskeletal structures. How this is regulated is not entirely clear but important to understand. While investigating Drosophila oogenesis, we found that the putative E3 ubiquitin ligase, Mind bomb 2 (Mib2), is required to promote epithelial stability and the collective cell migration of border cells. Through biochemical analysis, we identified components of Mib2 complexes, which include E-cadherin and α- and β-catenins, as well as actin regulators. We also found that three Mib2 interacting proteins, RhoGAP19D, Supervillin, and Myosin heavy chain-like, affect border cell migration. mib2 mutant main body follicle cells have drastically reduced E-cadherin-based adhesion complexes and diminished actin filaments. We conclude that Mib2 acts to stabilize E-cadherin-based adhesion complexes and promote a robust actin cytoskeletal network, which is important for maintenance of epithelial integrity. The interaction with cadherin adhesion complexes and other cytoskeletal regulators contribute to its role in collective cell migration. Since Mib2 is well conserved, it may have similar functional significance in other organisms.